Hamilton GlucoSense In-Situ Optical Glucose Sensor for Bioreactors

The Hamilton GlucoSense is an optical sensor that measures glucose directly inside mammalian cell culture bioreactors, in real time and without taking samples. It uses ATR mid-infrared (MIR) absorption, measures 0.5 to 25.0 g/L and reaches ±0.5 g/L after product calibration at the measurement temperature. It needs no enzymes, reagents, fiber optics or external spectrometer, fits existing PG 13.5 bioreactor ports and connects to the control system through its Arc RS485 Modbus output. As one of Hamilton’s distributors in Turkey, Mikron Proses (Üsküdar, Istanbul) supplies GlucoSense sensors and provides application consulting on sensor selection by port length and on ArcAir and Modbus integration.

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How it measures

GlucoSense measures the glucose concentration inside the reactor with a solid-state infrared optical sensor, so it removes the need for external spectrometers and complex calibrations. Calibration constants and diagnostic data are stored in the sensor head, and the sensor works with the Hamilton Arc peripherals and the ArcAir software; see the Arc peripherals and ArcAir page. Hamilton states that automated glucose control supports increased bioprocess productivity, optimized feeding strategies and improved consistency across development and manufacturing-scale bioreactors.

Sterilization and installation

The sensor can be autoclaved at 130 °C for 30 minutes together with the bioreactor. Hamilton’s first release targets wet-autoclave workflows with glass and benchtop bioreactors; for steam-in-place (SIP) stainless steel reactors, confirm suitability for your sensor version with the manufacturer.

Property	Value
Measurement principle	ATR mid-infrared (MIR), solid state, no fiber optics
Range / accuracy	0.5 to 25.0 g/L / ±0.5 g/L after product calibration
Output	Arc: RS485 Modbus RTU, VP8 connector
Sterilization	Autoclavable at 130 °C, 30 minutes
Process connection and lengths	PG 13.5 (12 mm diameter); 120, 160, 225, 325, 425 mm; titanium wetted parts with DLC coating

Combination with other Hamilton sensors

In a bioreactor, GlucoSense can share the Modbus line with the InCyte and Dencytee cell density sensors, the CO2NTROL dissolved CO2 sensor, the Hamilton dissolved oxygen sensors and the EasyFerm Plus pH sensors.

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Which shaft length fits your bioreactor depends on the port and the immersion depth. Tell us about your bioreactor and we will help you select the right model.